个人简介：

李成浩，男，农学博士，教授，博士生导师，林木遗传育种国家重点实验室“杨树适应性生长的遗传基础”研究组长；主要从事林木分子育种研究方向；重点研究林木不定根形成的分子调控机理、在干旱和氮磷亏缺等逆境条件下杨树根系可塑性发育的分子机理，以及落叶松基因工程育种研究。主要以杨树和落叶松为材料，重点研究林木不定根形成的分子调控机理、在干旱和氮磷亏缺等逆境条件下杨树根系可塑性发育的分子机理；鉴定、克隆调控林木根系发育和抗逆等重要目标性状的关键基因，解析其分子机制；并把鉴定出的关键调控基因应用到杨树和落叶松基因工程育种中；开展大青杨和落叶松的非转基因基因编辑体系建立和开展大青杨和落叶松非转基因基因编辑优良新品种培育研究。

主持“十四五”国家重点研发计划课题、国家自然科学基金面上项目、科技创新2030-“农业生物育种”重大项目子课题、国家转基因重大专项子课题等；获黑龙江省科技进步二等奖1项（第二名）；被授权国家发明专利10项；以第一作者或通讯作者在Plant Biotechnology Journal、New Phytologist、Plant Physiology和Journal of Integrative Plant Biology等植物科学领域高水平期刊发表论文多篇，在科学出版社和东北林业大学出版社出版专著2部。截至目前指导毕业博士9人，硕士30余人。

学习经历：

2001/02-2004/02，韩国江原大学，农业生命科学学院，农学学科，博士

1999/02-2001/02，韩国江原大学，农业生命科学学院，资源植物学科，硕士

1989/09-1993/07，延边农学院，果林系，果树专业，学士

工作经历：

2009/09-至今，东北林业大学，林学院林木遗传育种学科，教授、博导

2004/02-2009/08，东北林业大学，林学院林木遗传育种学科，副教授、硕导

2004/10-2007/10，东北林业大学，博士后，合作导师：杨传平

1993/07-1999/02，吉林省延边华龙集团，龙井果树农场，技术员

代表性成果：

项目：

1. 国家自然科学基金面上项目，32371899，大青杨PuHox52协同碳氮代谢调控木材形成的分子机制研究，2024.01-2027.12，主持

2. “十四五”国家重点研发计划课题，2023YFD2200205，树木干细胞对逆境胁迫的响应机制，2023.12-2028.11，主持

3. 科技创新2030-“农业生物育种”重大项目子课题，2022ZD040160203，落叶松速生高抗新种质的创制、选择、评价和示范推广，2022/12-2025/12，主持

4. 科技创新2030-“农业生物育种”重大项目任务，2023ZD040580301抗松毛虫高产落叶松新种质创制及选育2023/09-2025/12，主持

5. 国家自然科学基金面上项目，31971671，大青杨PuMYB40调控低磷诱导不定根发生和低磷胁迫抗性的分子机制，2020.01-2023.12，主持

6. 国家自然科学基金面上项目，31670668，毛果杨HD-Zip转录因子调控不定根发生和低氮胁迫抗性的分子机理研究，2017.01-2020.12，主持

7. 转基因生物新品种培育重大专项课题任务，2018ZX08020003-001-001，转基因落叶松新品种培育及产业化研究，2018.01-2020.12，主持

8. 中央高校基本科研业务费重大项目培育专项，2572018CL04，基于CRISPR系统的非转基因针叶树和阔叶树基因组编辑技术体系的建立，2018.06-2021.12，主持

9. 林木遗传育种国家重点实验室创新项目，2020A02，PuHox52在大青杨根发育和低氮胁迫响应中的转录和表观遗传调控机制研究，2020.06-2022.12，主持

10. 国家重点研发专项项目任务，2017YFD0600603-0105，白桦良种体胚规模化繁育技术体系，2017.07-2020.12，主持

11. 国家高新技术研究发展计划（863计划）项目子课题，2013AA102704-02，落叶松抗逆基因工程研究，2013.01-2017.12，主持

12. 国家高新技术研究发展计划（863计划）项目子课题，2011AA100202-1-4，白桦BpMYB168基因克隆和速生、高光效白桦分子育种，2011.01-2015.12，主持

13. 国家高新技术研究发展计划（863计划）项目子课题，2011AA100203，长白落叶松分子育种及品种创制研究，2011.01-2015.12，主持

14. 中央高校基本科研业务费创新团队与重大项目培育资金项目，DL11EA02，柳树和白桦抗逆、优质基因挖掘和分子育种研究，2011.10-2014.10，主持

15. 国家自然科学基金面上项目，30671701，刺五加体细胞胚在生物反应器中的生长动力学及抗氧化特性，2007.01-2009.12，主持

16. 东北林业大学高层次人才引进项目，刺五加体细胞胚的生物反应器培养研究，2004.04-2008.12，主持

论文：

1. Ming Wei, Mengqiu Zhang, Jiali Sun, Ying Zhao, Solme Oak, Miaomiao Ma, Yingxi Chen, Han Lu, Jingli Yang, Hairong Wei, Yuhua Li,Chenghao Li*. PuHox52 promotes coordinated uptake of nitrate, phosphate, and iron under nitrogen deficiency in Populus ussuriensis.Journal of Integrative Plant Biology, 2023, 65: 791-809. (SCI, IF=10.1)

2. Hanzeng Wang, Solme Pak, Jia Yang, Ye Wu, Wenlong Li, He Feng, Jingli Yang, Hairong Wei*,Chenghao Li*. Two high hierarchical regulators, PuMYB40 and PuWRKY75, control the low phosphorus driven adventitious root formation in Populus ussuriensis.Plant Biotechnology Journal, 2022, (SCI, IF=9.803)

3. Dandan Li, Jingli Yang, Solme Pak, Minzhen Zeng, Jiali Sun, Sen Yu, Yuting He,Chenghao Li*. PuC3H35 confers drought tolerance by enhancing lignin and proanthocyanidin biosynthesis in the roots of Populus ussuriensis.New Phytologist, 2022, 233:390-408. (SCI, IF=10.151)

4. Quangang Liu, Zhanchao Wang, Sen Yu, Wenlong Li, Mengqiu Zhang, Jia Yang, Dandan Li, Jingli Yang*,Chenghao Li*. Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar.Journal of Experimental Botany, 2021, 72:1370-1383. (SCI, IF=6.992)

5. Ming Wei, Quangang Liu, Zhanchao Wang, Jingli Yang, Wenlong Li, Yingxi Chen, Han Lu, Jinfu Nie, Baoguang Liu, Kaiwen Lv, Xuliang Mao, Su Chen, Jennifer Sanders, Hairong Wei*,Chenghao Li*. PuHox52-mediated hierarchical multilayered gene regulatory network promotes adventitious root formation in Populus ussuriensis.New Phytologist, 2020, 228:1369-1385. (SCI, IF=10.151)

6. Haizhen Zhang, Jingli Yang, Wenlong Li, Yingxi Chen, Han Lu, Shicheng Zhao, Dandan Li, Ming Wei,Chenghao Li*. PuHSFA4a enhances tolerance to excess zinc by regulating reactive oxygen species production and root development in Populus.Plant Physiology, 2019, 180:2254-2271. (SCI, IF=8.34)

7. Miaomiao Ma, Chan Zhang, Lijing Yu, Jingli Yang,Chenghao Li*. CRISPR/Cas9 ribonucleoprotein mediated DNA-free genome editing in larch.Forestry Research, 2024.4: e036.

8. Lu Li, Xi Ren, Liying Shao, Xun Huang, Chunyan Zhang, Xuhui Wang , Jingli Yang,Chenghao Li*. Comprehensive analysis of the NF-YB gene family and expression under abiotic stress and hormone treatment in Larix kaempferi.Int. J. Mol. Sci, 2023, 24, 8910. (SCI, IF=4.9)

9. Miaomiao Ma, Xuhui Wang, Chan Zhang, Solme Pak, Hongran Wu, Jingli Yang,Chenghao Li*. Enhancing the cryopreservation system of larch embryogenic culture by optimizing pre-Culture, osmoprotectants, and rapid thawing.Forests, 2023, 14(8), 1621. (SCI, IF=2.4)

10. Solme Pak,Chenghao Li*. Progress and challenges in applying CRISPR/Cas techniques to the genome editing of trees.Forestry Research, 2022, 2:6.

11. Wenlong Li, Zhiwei Liu, He Feng, Jingli Yang andChenghao Li*. Characterization of the gene expression profile response to drought stress in Populus ussuriensis using PacBio SMRT and illumina sequencing.International Journal of Molecular Sciences, 2022, 23:3840. (SCI, IF=5.923)

12. Jia Yang, Ye Wu, Lu Li,Chenghao Li*. Comprehensive analysis of the BES1 gene family and its expression under abiotic stress and hormone treatment in Populus trichocarpa.Plant Physiology and Biochemistry, 2022, 173:1-13. (SCI, IF=4.27)

13. Yanan Jing, Yue Yu, Hanzeng Wang, Ye Wu,Chenghao Li*. Genome‑wide identification and expression analysis of the bZIP gene family in silver birch (Betula pendula Roth.).Journal of Forestry Research,2022, https://doi.org/10.1007/s11676-022-01453-w. (SCI, IF=2.149)

14. Qiurong Ren, Simon Sretenovic, Shishi Liu, Xu Tang, Lan Huang, Yao He, Li Liu, Yachong Guo, Zhaohui Zhong, Guanqing Liu, Yanhao Cheng, Xuelian Zheng, Changtian Pan, Desuo Yin, Yingxiao Zhang, Wanfeng Li, Liwang Qi,Chenghao Li, Yiping Qi*, Yong Zhang*. PAM-less plant genome editing using a CRISPR-SpRY toolbox.Nature Plants, 2021, 7:25-33. (SCI)

15. Wenlong Li, Joobin Lee, Sen Yu, Fude Wang, Wanqiu Lv, Xin Zhang,Chenghao Li*, Jingli Yang*. Characterization and analysis of the transcriptome response to drought in Larix kaempferi using PacBio full length cDNA sequencing integrated with de novo RNA seq reads.Planta, 2021, 253:28. (SCI, IF=4.116)

16. Jingli Yang, Wanqiu Lv, Liying Shao, Yanrui Fu, Haimei Liu, Chengjun Yang, Aihua Chen, Xieyu Xie, Zhiwei Wang,Chenghao Li*. PacBio and Illumina RNA sequencing identify alternative splicing events in response to cold stress in two poplar species.Frontiers in Plant Science, 2021, 12:737004. (SCI, IF=5.753)

17. Jingli Yang, Hanzeng Wang, Shicheng Zhao, Xiao Liu, Xin Zhang, Weilin Wu,Chenghao Li*. Overexpression levels of LbDREB6 differentially affect growth, drought, and disease tolerance in poplar.Frontiers in Plant Science, 2020, 11:528550. (SCI, IF=5.753)

18. Dandan Li, Sen Yu, Minzhen Zeng, Xiao Liu, Jia Yang,Chenghao Li*. Selection and validation of appropriate reference genes for real-time quantitative PCR analysis in needles of Larix olgensis under abiotic stresses.Forests, 2020, 11:193. (SCI, IF=2.633)

19. Ming Wei, Yingxi Chen, Mengqiu Zhang, Jingli Yang, Han Lu, Xin Zhang,Chenghao Li*. Selection and validation of reference genes for the qRT-PCR assays of Populus ussuriensis gene expression under abiotic stresses and related ABA treatment.Forests, 2020, 11:476. (SCI, IF=2.633)

20. Hanzeng Wang, Xue Leng, Xuemei Xu,Chenghao Li*. Comprehensive analysis of the TIFY gene family and its expression profiles under phytohormone treatment and abiotic stresses in roots of Populus trichocarpa.Forests, 2020, 11:315. (SCI, IF=2.633)

专著：

1. 李成浩，杨静莉，张海珍著。大青杨抗逆基因工程育种。科学出版社，2022年。

2. 李成浩著。红皮云杉和杂种落叶松离体培养再生体系的建立研究。东北林业大学出版社，2009年。

奖励：

1. 张含国，李成浩等。杂种落叶松优良家系选育与扩繁技术，2013年，黑龙江省科技进步二等奖。

联系方式：

邮箱：chli@nefu.edu.cn; chli0@163.com

Resume

Chenghao Li, Ph.D. in Agriculture, Professor, Ph.D.’s Supervisor.

Research area:

(1) Transcriptional and epigenetic regulation mechanisms of woody plants in response to drought and nitrogen/phosphorus deficiency

(2) Mining stress-resistant genes and genetic engineering breeding in poplar and larch

(3) Optimization of somatic embryogenesis and plant regeneration processes in woody and medicinal plants

(4) Establishment of tissue culture regeneration and transgenic systems for recalcitrant tree species

Email:chli@nefu.edu.cn; chli0@163.com